bip grp78 inhibitor  (MedChemExpress)

Journal: iScience

Article Title: Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish

doi: 10.1016/j.isci.2023.108207

Figure Lengend Snippet: BAF60c interacts with GRP78 (A) Co-IP of HEK293T cells overexpressing BAF60c-HA and GRP78-FLAG. (B) HEK293T cells were co-transfected with BAF60c-GFP and GRP78-RFP plasmids for 24 h and followed by laser scanning confocal microscopy to observe the colocalization of BAF60c and GRP78. Scale bars, 20 μm.

Article Snippet: GRP78 agonist (Bip inducer X; MCE) and GRP78 inhibitor (HM03; MCE) were incubated with a concentration of 10 μM for different time points.

Techniques: Co-Immunoprecipitation Assay, Transfection, Confocal Microscopy

Journal: iScience

Article Title: Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish

doi: 10.1016/j.isci.2023.108207

Figure Lengend Snippet: BAF60c regulates GRP78 expression in fish (A) Relative mRNA expression of BAF60c and GRP78 after BAF60c-siRNA treatment for 36 h in macrophage. (B) Relative mRNA expression of BAF60c and GRP78 after BAF60c-siRNA treatment for 36 h in hepatocyte. (C) Relative mRNA expression of BAF60c and GRP78 after overexpression of BAF60c for 24 h in hepatocyte. (D) Relative mRNA expression of BAF60c and GRP78 after BiP treatment for 4 h with different concentrations in macrophages. (E) Protein level of BAF60c after 10 μM BiP treatment for 4 and 8 h in macrophages. (F) Relative mRNA expression of BAF60c and GRP78 after HM03 treatment for 4 h with different concentrations in macrophages. (G) Protein level of BAF60c after 10 μM HM03 treatment for 3 and 5 h in macrophages. Data are represented as mean ± SEM (n = 3) and were analyzed using independent t test. The “∗” means a significant difference (p < 0.05), and “∗∗” means a highly significant difference (p < 0.01).

Article Snippet: GRP78 agonist (Bip inducer X; MCE) and GRP78 inhibitor (HM03; MCE) were incubated with a concentration of 10 μM for different time points.

Techniques: Expressing, Over Expression

Journal: iScience

Article Title: Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish

doi: 10.1016/j.isci.2023.108207

Figure Lengend Snippet: Knockdown of GRP78 in macrophages and hepatocytes can reduce ER stress, inflammation, and fatty acid biosynthesis (A) Relative mRNA expression of grp78 after GRP78-siRNA treatment for 36 h in macrophages. (B) Relative mRNA expression of ER stress-related genes after GRP78-siRNA treatment for 36 h in macrophages. (C) Relative mRNA expression of inflammatory genes after GRP78-siRNA treatment for 36 h in macrophages. (D) Relative mRNA expression of grp78 after GRP78-siRNA treatment for 36 h in hepatocytes. (E) Relative mRNA expression of ER stress-related genes after GRP78-siRNA treatment for 36 h in hepatocytes. (F) Relative mRNA expression of inflammatory genes after GRP78-siRNA treatment for 36 h in hepatocytes. (G) Relative mRNA expression of lipid metabolism-related genes after GRP78-siRNA treatment for 36 h in hepatocytes. Data are represented as mean ± SEM (n = 3) and were analyzed using independent t test. The “∗” means a significant difference (p < 0.05), and “∗∗” means a highly significant difference (p < 0.01).

Article Snippet: GRP78 agonist (Bip inducer X; MCE) and GRP78 inhibitor (HM03; MCE) were incubated with a concentration of 10 μM for different time points.

Techniques: Knockdown, Expressing

Journal: iScience

Article Title: Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish

doi: 10.1016/j.isci.2023.108207

Figure Lengend Snippet:

Article Snippet: GRP78 agonist (Bip inducer X; MCE) and GRP78 inhibitor (HM03; MCE) were incubated with a concentration of 10 μM for different time points.

Techniques: Virus, Recombinant, Transfection, Sequencing, Plasmid Preparation, Software

Journal: Biomedicines

Article Title: Role of UPR Sensor Activation in Cell Death–Survival Decision of Colon Cancer Cells Stressed by DPE Treatment

doi: 10.3390/biomedicines9091262

Figure Lengend Snippet: BiP is upregulated in the fraction of cells resistant to the DPE treatment. ( A ) RKO and SW480 were treated with DPE (50 or 100 μM) or left untreated as controls. After 48 h, the expression of BiP in living cells or dead cells was evaluated via Western blot analysis. β-Actin was used as the loading control. The histograms represent the means plus S.D. from the densitometric analysis of the ratio between BiP and β-actin. ( B ) The cell viability levels of RKO and SW480 cell lines pre-treated with HA15 (10 μM) and then treated with DPE (50 μM) or left untreated as control (CT) were measured via trypan blue exclusion assay after 48 h of culture. ( C ) RKO and SW480 were pre-treated with HA15 (10 μM) and then treated with DPE (50 μM) or left untreated as control. After 48 h, the expression levels of BiP, CHOP, and Mcl-1 were evaluated via Western blot analysis. β-Actin was used as the loading control. The histograms represent the means plus S.D. from the densitometric analysis of the ratio between the protein and β-actin. Note: ** p < 0.01, * p < 0.05.

Article Snippet: Some experimental cells were plated in 12-well plates, as reported above, then the day after were pre-treated with HA15 (BiP/GRP78 inhibitor) (Sigma-Aldrich, MO, USA, SML2118), 4μ8C (IRE1 RNAse inhibitor) (Sigma-Aldrich, Saint Louis, MO, USA, SML0949), ceapin-A7 (ceapin) (ATF6a signaling blocker) (Sigma-Aldrich, MO, USA, SML2330), or GSK2606414 (GSK) (PERK inhibitor) (Selleckem, Houston, TX, USA, S7307).

Techniques: Expressing, Western Blot, Trypan Blue Exclusion Assay